Tritium sequence analysis of polyribonucleotides following periodate-phosphomonoesterase degradation - characterization of oligonucleotide-3′ dialdehyde intermediates
نویسندگان
چکیده
منابع مشابه
Sequence analysis of nonradioactive RNA fragments by periodate-phosphatase digestion and chemical tritium labeling: characterization of large oligonucleotides and oligonucleotides containing modified nucleosides.
A tritium derivative method for sequence analysis of polyribonucleotides is detailed, which is based on borotritide reduction of oligonucleotide dialdehydes generated by treatment of polyribonucleotides with alkaline phosphatase and excess periodate at pH 8 (borate buffer; no primary amine present in the reaction mixture). While neither phosphatase nor periodate possess any intrinsic exonucleas...
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A tritium derivative method for sequence analysis of polyribonucleotides is detailed, which is based on borotritide reduction of oligonucleotide-3' dialdehydes generated by controlled snake venom phosphodiesterase/alkaline phosphomonoesterase digestion and periodate treatment of time point aliquots of the incubation mixture. Radioactive oligonucleotide derivatives are resolved according to chai...
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Whitfeld and Markham (1) described a method for the stepwise degradation of polyribonucleotides which involved oxidation of the vicinal hydroxyl groups of the terminal nucleoside residue to a dialdehyde by means of periodate, followed by treatment with glycine at pH 10.5 to remove the terminal base. In this way they converted GpC to 3’-GNP and cytosine. A similar procedure was applied to 5’-AMP...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1974
ISSN: 0014-5793
DOI: 10.1016/0014-5793(74)80924-5